Factors affecting the rate of enzyme

What are the Factors that Affect the Enzymatic Activity? Several factors affect the rate of an enzyme catalyzed reaction.

Factors affecting the rate of enzyme

Factors Affecting Enzyme Activity: This article throws light upon the six factors affecting the enzyme activity. The six factors are: The contact between the enzyme and substrate is the most essential pre-requisite for enzyme activity.

Factors affecting the rate of enzyme

The important factors that influence the velocity of the enzyme reaction are discussed hereunder: As the concentration of the enzyme is increased, the velocity of the reaction proportionately increases Fig. In fact, this property of enzyme is made use in determining the activities of serum enzymes for diagnosis of diseases.

Increase in the substrate concentration gradually increases the velocity of enzyme reaction within the limited range of substrate levels.

A rectangular hyperbola is obtained when velocity is plotted against the substrate concentration Fig. Three distinct phases of the reaction are observed in the graph. Enzyme kinetics and Km value: The enzyme E and substrate S combine with each other to form an unstable enzyme-substrate complex ES for the formation of product P.

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Here k1, k2 and k3 represent the velocity constants for the respective reactions, as indicated by arrows. It indicates that half of the enzyme molecules i. Km value is a constant and a characteristic feature of a given enzyme.

It is a representative for measuring the strength of ES complex. A low Km value indicates a strong affinity between enzyme and substrate, whereas a high Km value reflects a weak affinity between them.

For majority of enzymes, the Km values are in the range of to moles. Line weaver-Burk double reciprocal plot: For the determination of Km value, the substrate saturation curve Fig.

By taking the reciprocals of the equation 1a straight line graphic representation is obtained. The Line weaver-Burk plot is shown in Fig.There are many factors that contribute to the rate of reaction of an enzyme.

Factors include: concentration of the enzyme, temperature, pH level, concentration of the substrate, and inhibitors. This lab shows the affects these factors have on the rate of reaction between catalase, an enzyme found in potatoes, and hydrogen peroxide, the substrate.

AP Biology Lab Factors Influencing Enzyme Activity. AP Biology Factors Influencing Enzyme Activity Lab By: Chase, Brian, Walter, and Nick The General Questions: 1. Balanced Equation: 2 H2O>2 H2O + O2 2. What would cause the rate of enzyme activity to level off on the substrate concentration graph? 2.) The enzyme reaching its point of. Summary of Factors That Affect Chemical Reaction Rate The chart below is a summary of the main factors that influence reaction rate. Keep in mind, there is typically a maximum effect, after which changing a factor will have no effect or will slow a reaction. Factors Affecting The rate of Enzyme Activity Prediction: As the temperature increases the rate of enzyme activity will also increase, thus increasing the rate of reaction. However, if the temperature is too high the enzyme will denature.

Apr 14,  · Factors that affect enzyme catalyzed reactions Substrate concentration, enzyme concentration, temperature and pH affect the rate of an enzyme catalyzed reaction. Substrate concentration- the rate of reaction increases with substrate concentration until a .

Several factors affect the rate of an enzyme catalyzed reaction. Among these are enzyme concentration, substrate concentration, temperature and pH. Besides, some molecules (activators) increase enzyme activity, while others (inhibitors) retard enzymatic activity.

Factors Affecting Enzyme Activity (Introduction to Enzymes)

Apr 14,  · Factors that affect enzyme catalyzed reactions Substrate concentration, enzyme concentration, temperature and pH affect the rate of an enzyme catalyzed reaction. Substrate concentration- the rate of reaction increases with substrate concentration until a .

FACTORS AFFECTING ENZYME ACTIVITY PURDUE UNIVERSITY INSTRUMENT VAN PROJECT QUESTIONS 1. In what way is the rate of the reaction dependent on the concentration of the enzyme?

Factors affecting enzyme activity. Published December 6, Numerous factors affect the reaction rate.

Table of Contents Factors affecting Enzyme Activity Factors affecting Enzyme Activity The activity of an Enzyme is affected by its environmental conditions. Changing these alter the rate of reaction caused by the enzyme.

The Michaelis-Menten equation is a quantitative description of the relationship among the rate of an enzyme- catalyzed reaction [v 1], the concentration of substrate [S].

BBC - Standard Grade Bitesize Biology - Enzymes and aerobic respiration : Revision, Page 5